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High Throughput Sequencing Core Services
HTS Core provides access to "Next Generation" short-read sequencing on Illumina platforms and "Third Generation" or long-read on Oxford Nanopore Technology (ONT). HTS Core provides consultation for experimental design, library preparation, and sequencing for a broad range of projects. We are here to listen and create solutions.
Consultation for project planning and study design
HTS Core offers consultation services for study design and choosing the most effective sequencing platform for the study.
Quality Control (QC) for DNA, RNA, customer-made library, etc.
We offer capillary electrophoresis devices for quality control of RNA and DNA samples. We support the use of RNA Nano, RNA Pico, genomic DNA, and High sensitivity DNA on Agilent Bioanalyzer1000 and Agilent Tape Station4000.
For the customer-made library, we offer quantitative Real-Time PCR on our QuantStudio Flex Real-Time PCR Systems.
Library preparation
- Whole Genome and Whole Exome
- Targeted Sequencing (Cancer or Custom Panels, minimum sample number required)
- Transcriptome sequencing (RNA-seq)
- Epigenome (Chip-seq, BS-seq, RRBS-seq, WGBS)
- miRNA sequencing
- Metagenomics
- ctDNA sequencing (NGS provides an excellent technology that allows the detection of low levels of ctDNA in the blood in a highly sensitive and specific manner)
We offer manual and automated library preparation services; sample requirement varies depending on the application. Please contact the Core for details.
Sequencing
We offer full-service and self-service sequencing on both Illumina and Oxford Nanopore platforms. The sequencing platform is chosen depending on the application and required coverage. FASTQ files are provided to the customer, as HTS Core does not provide data analysis services.