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In This Section
Deep Sequencing Accelerating CHOP and Penn Research
CHOP and Penn’s most innovative researchers are using deep sequencing available at the Center for Applied Genomics to accelerate their advanced research priorities. Hear how David Hill, MD, PhD, and Ruth-Ann Langan Pai have successfully leveraged single cell RNA-sequencing to explore pathogenic mechanisms in allergy and immunology research.
Learn how you can leverage high intensity sequencing and techniques available at the Center for Applied Genomics such as bulk and single-cell RNA-seq methods, their applications and analysis, multi-omics, and computational methods to accelerate your research.
Illumina and 10X Genomics product updates will be given, including exciting information surrounding Spatial Transcriptomics.
Opportunity for Complimentary Sequencing Service!
Do you have a research question that you’d like to explore as a pilot study using Single-Cell RNA sequencing, but do not have current funding to execute it?
The CAG Sequencing Core invites new customers to compete for a courtesy $5,000 Single-Cell RNAseq service (sequencing and data analysis) for the purposes of exploring a new research question. Simply submit a 300-word summary/abstract illustrating the potential impact of the pilot. CAG’s Renata Pellegrino, PhD, and Patrick Sleiman, PhD, will evaluate and award the two most compelling submissions.
- New CAG Customer!
- Attendance at the February 18 event
- Demonstration that the pilot study holds potential to provide the investigator future research funding
- Submit by Friday, February 28 and decisions will be communicated on or before March 20
Light Refreshments will be served.
Seating is limited!
Please RSVP before Friday, February 14 to Renata Pellegrino at silvar [at] email.chop.edu
2 p.m. – 2:15 p.m.: Meet and Greet
2:15 p.m. – 2:30 p.m.: Welcome and Introduction by Renata Pellegrino, PhD, CAG Technical Director
2:30 p.m. – 3:00 p.m.: “Successful employment of single cell RNA-sequencing to explore the role of pathogenic T cells in food allergy,” by David Hill, MD, PhD
3 p.m. – 3:20 p.m.: “Novaseq6000: Enable Deep Discovery by generating Big Data at greater affordability,” by Dan Gheba, Illumina
3:25 p.m. – 4 p.m.: “Single cell 10x Genomics single-cell RNA sequencing technology to explore Castleman disease biology and identify potential pathogenic mechanisms that can be targeted with existing therapeutics,” by Ruth-Ann Langan Pai, 4th Year PhD candidate, Immunology Graduate Group, Mentors: David Fajgenbaum, MD, and Dr. Taku Kambayashi
4 p.m. – 4:20 p.m.: “Visium Gene Expression Solution - Discover the Genes that Matter While Preserving Spatial Information,” by Kasandra Burgos PhD, 10X Genomics
4:20 p.m. – 4:30 p.m.: RNA Sequencing Competition Submission Guidelines and Closing Remarks, by Dr. Pellegrino