Human Pluripotent Stem Cell Core

The pluripotent stem cell and genome editing technologies represent exciting new tools for studying human disease. Having unlimited supplies of cells that either express or are genetically manipulated to express genes of interest provides investigators with human model systems to study disease mechanisms and identify new therapies.

The Core generates induced pluripotent stem cell (iPSC) lines from somatic cells including peripheral blood mononuclear cells, fibroblasts, and lymphoblastoid cell lines. These lines are generated using the latest non-integrating reprogramming methodologies that leave the genome intact. The Core has established standard operating procedures for:

• Pluripotent stem cell growth and maintenance
• Genome editing technologies including CRISPR-CAS9
• Differentiation to germ layer and derivative tissues of interest.

The Core provides enrichment training courses for investigators interested in learning how to work with pluripotent stem cells and/or establish the pluripotent stem cell technology in their own labs.

The Human Pluripotent Stem Cell Core, established in 2008 by the Raymond G. Perelman Center for Cellular and Molecular Therapeutics at Children’s Hospital of Philadelphia, serves the research needs of CHOP, the University of Pennsylvania, and outside academic communities in the field of human pluripotent stem cell biology.