About Coulter Laboratory

Our laboratory has focused our energies on dynamic characterization of the function of neuronal circuits, integrating a combination of techniques to fully interrogate all aspects of brain network behavior, both in control and in models of epilepsy.

We have three, two-photon microscopy systems, a swept-field fast confocal system, a laser scanning confocal system, voltage sensitive dye imaging systems, and electrophysiology microscopy systems. Each rig is customized for performing different type of experiments.

Technical approaches utilized in the laboratory include:

• Functional epifluorescence imaging of transmembrane voltage changes in populations of neurons
• Multiphoton, confocal, and miniscope imaging of intracellular calcium dynamics both in individual neurons and large populations of neurons
• Fluorescence lifetime and multiphoton imaging of transmembrane chloride dynamics both in individual neurons and in populations of neurons
• Multielectrode array recording of neuronal activation within hippocampal microcircuits
• Transgenic labeling and gene targeting in specified subpopulations of neurons
• Optogenetic activation and silencing of specified populations of neurons
• Patch clamp and extracellular recording techniques