High Throughput Sequencing Core FAQs

Please support us by acknowledging our services in your publications. Please acknowledge Core staff where appropriate and consider authorship inclusion when scientific contributions merit. Please feel free to use the following language in your acknowledgments. Thank you in advance!

"Sequencing was performed at CHOP High Throughput Sequencing Core."

"We thank High Throughput Sequencing Core at CHOP for their help with sequencing."

HTS Core houses specialized equipment for DNA and RNA sequencing projects including Illumina Nextseq1000, NovaSeq6000 and ONT PromethION24 sequencers. Agilent 2100 Bioanalyzer and 4200 Tape Station, Qubit 2.0 Fluorometer, and several Real-Time qPCR Detection Systems for sample quality, and quantity assessment. High throughput sequencing (e.g., whole genome/exome, targeted sequencing, transcriptome, ATAC-seq, ChIP-seq, miRNA, metagenome) and variant detection are only a few of the services provided. HTS Core further offers sequencing-associated services such as library preparation and QC, and project design consultations. HTS Core offers many RNA-seq library prep options such as microRNA, ribosomal RNA-depletion, polyA enrichment with strand-specific sequencing, and ultra-low input library preparation.

Fees are available in iLab.

Please note HTS Core does not provide data analysis services.

HTS Core does not perform DNA/RNA purification. Many well-established commercial kits and protocols exist for a variety of species and tissue/cell types. Investigators need to determine the most appropriate methods for their cell/tissue type.

For RNA samples, we require DNase treatment prior to sample submission.

Ideally, we would like an aliquot of 5 ul of DNA/RNA to perform sample QC; 3 ul is required.

Samples may be dropped off between 8:30 a.m. and 3:30 p.m., Please contact the Core staff for sample submission only after an iLab request has been received.

Please do not bring samples without an appointment.