The Center for Applied Genomics Core

The Center for Applied Genomics Core has an experienced team of researchers analyzing the immense amount of data generated from our high-throughput facility, with expertise in carrying out large scale NGS, GWAS, CNV, and methylation studies. Currently at CAG, there are 50 active disease projects, both internal and collaborative. CAG is collaborating with investigators at The Children's Hospital of Philadelphia, the University of Pennsylvania, as well as with international investigators from Croatia, United Kingdom, Denmark and Canada, making their genotyping an international endeavor.

Bioinformatics

The Genome Analysis and Bioinformatics group of the Center for Biomedical Informatics provides bioinformatics capabilities to help researchers effectively manage, analyze and use their experimental data, resulting in the enhancement and empowerment of research, discovery, and clinical application. Services provided include next-generation sequencing processing and analysis; genomic, functional genomic, and protein data analysis in collaboration with investigators; large data set manipulation and management; data visualization; high-performance computing; application and database access, usage and implementation; and web-based data dissemination solutions.

Research Viral Vector

The Research Viral Vector Core: As part of The Center for Cellular and Molecular Therapeutics, the Research Vector Core (RVC) has expanded its capacity to provide infrastructure support for investigators interested in using viral vectors in their research model systems.

Clinical Viral Vector

The Clinical Vector Core utilizes a patented vector production technology and a highly efficient purification process that utilizes combined column and gradient centrifugation-based process steps. This system has manufactured clinical grade AAV vectors that have demonstrated excellent safety in several clinical studies.

hESC/iPSC

The human embryonic stem cell/induced pluripotent Stem Cell (hESC/iPSC) Core generates control and patient-specific iPSC lines by cellular reprogramming using current technologies. The core has a training course in cell maintenance and growth, specialized differentiation protocols for disease modeling and drug screening, and quality-control reagents for the Children’s Hospital and University of Pennsylvania academic communities. The facility maintains five NIH-approved human ESC lines and has generated more than 60 disease-specific iPSC lines.

Healthcare Analytics

The Healthcare Analytics Unit (HAU) serves as a resource for Hospital investigators using administrative data to address research questions by providing expertise in analysis data creation, statistical programming, advanced analysis, study design, and sample size calculation. The HAU is a core service unit of two centers in the CHOP Research Institute – the Center for Pediatric Clinical Effectiveness (CPCE) and PolicyLab. CPCE performs clinical epidemiology, health services, and community-based research to elucidate the best practices in the diagnosis, prognosis, and management of pediatric disease. PolicyLab researchers are methodological experts in the disciplines of epidemiology, health services research, program evaluation, and ethics.

CIRP Simulator Program

The Center for Injury Research and Prevention (CIRP) advances the safety and health of children, adolescents, and young adults through comprehensive research resulting in practical tools to reduce injury and promote recovery. One of our focus areas is young driver behavior. The Simulator Program, located within CIRP, provides the technical and administrative support for conducting simulator-based observational studies.

The Simulator Program features a three-channel, open-cockpit-configured driving simulator with a driver’s seat; a one-channel, desktop driving simulator with a game control console; an eye-tracking system that is integrated with the open-cockpit driving simulator; video-based data visualization program for processing simulator data; and a specialty program for analyzing eye-tracking data. CIRP’s current simulator-based projects include examination of the effects of distraction and peer passenger on teen drivers’ behaviors, development of standardized driving assessment protocols, and evaluations of web-based interventions and curriculum.