Gerd
 
A.
 
Blobel
MD, PhD
Email: 
blobel@email.chop.edu
Address: 
316H Abramson Research Center 3615 Civic Center Blvd.
(215) 590-3988
Affiliations
Expertise

Research Interests
hematopoiesis, higher order chromatin structure, gene expression, epigenetic memory, transcription factors

Description of Research
Our lab focuses on the genetic and epigenetic control of hematopoiesis and its disorders. Specifically, we study how tissue-specific transcription factors govern the specification and maintenance of hematopoietic cell lineages. We also examine how transcription programs are epigenetically transmitted through mitosis to maintain lineage identity. Our work is leading us deeply into the analysis of chromatin structure, its modifications and organization. For example, we investigate how regulatory elements are organized spatially using chromosome conformation capture technology. We are also interested in the control of transcription factors by posttranslational modifications. For our studies we combine molecular, genomic, biochemical and imaging approaches with studies in normal and gene targeted mice.

Lab Rotation Projects:
1) Regulation of higher order chromatin organization during gene activation and repression
2) Mechanisms of epigenetic bookmarking during mitosis
3) Lineage choice decisions by GATA1, FOG1 and Ets transcription factors during hematopoiesis
4) Molecular mechanisms of GATA1 acetylation
5) Linking GATA1 mutations to molecular disease pathways

Lab personnel:
Amy Campbell, graduate student
Chris Hsiung, combined degree student
Sarah Hsu combined degree student
Aaron Stonestrom, combined degree student
Rena Zheng, combined degree student
Wulan Deng, postdoctoral fellow
Chris Edwards, postdoctoral fellow
Peng Huang, postdoctoral fellow
Kiwon Lee, postdoctoral fellow
Jeremy Rupon, postdoctoral fellow
Suzi Shapira rotation student
Hongxin Wang, technician/lab manager

Appointments
Assistant Professor of Pediatrics at University of Pennsylvania School of Medicine (1997 – 2003)
Associate Professor of Pediatrics at University of Pennsylvania School of Medicine (2003 – 2008)
Professor of Pediatrics at University of Pennsylvania School of Medicine (2008– present)
Education
Selected Publications
Jing H, Vakoc CR, Ying L, Mandat S, Wang H, Zheng X, Blobel GA. Exchange of GATA factors mediates transitions in looped chromatin organization at a developmentally regulated gene locus.. Molecular Cell. Vol 29. 2008:232.
Blobel GA, Kadauke S, Wang E, Lau AW, Zuber J, Chou MM, Vakoc CR. A reconfigured pattern of MLL occupancy within mitotic chromatin promotes rapid transcriptional reactivation following mitotic exit.. Molecular Cell. Vol 36. 2009:970.
Gregory GD, Miccio A, Bersenev A, Wang Y, Hong W, Zhang Z, Poncz M, Tong W, Blobel GA. FOG1 requires NuRD to promote hematopoiesis and maintain lineage fidelity within the megakaryocytic-erythroid compartment.. Blood. Vol 115. 2010:2156.
Miccio A, Blobel GA. The role of the GATA-1/FOG-1/NuRD pathway in the expression of human {beta}-like globin genes.. Mol. Cell. Biol.. Vol 30. 2010:3460.
Deng W, Blobel GA. Do chromatin loops provide epigenetic gene expression states?. Curr. Opin. Genet. Dev.. Vol 20. 2010:548.
Miccio A, Wang Y, Hong W, Gregory GD, Wang H, Yu X, Choi JK, Shelat S, Tong W, Poncz M, Blobel GA. NuRD mediates activating and repressive functions of GATA-1 and FOG-1 during blood development.. EMBO J.. Vol 29. 2010:442.
Gamsjaeger R, Webb SR, Lamonica JM, Billin A, Blobel GA, Mackay JP. Structural Basis and Specificity of Acetylated Transcription Factor GATA1 Recognition by BET Family Bromodomain Protein Brd3.. Mol. Cell. Biol.. Vol 31. 2011:2632.
Lamonica JM, Deng W, Kadauke S, Campbell AE, Gamsjaeger R, Wang H, Cheng Y, Billin AN, Hardison RC, Mackay JP, Blobel GA. Bromodomain protein Brd3 associates with acetylated GATA1 to promote its chromatin occupancy at erythroid target genes.. PNAS. Vol 108. 2011:159.
Deng W, Lee J, Wang H, Miller J, Reik A, Gregory PD, Dean A, Blobel GA. Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor. Cell. Vol 149. 2012:1233.
Kadauke S, Udugama MI, Pawlicki JM, Achtman JC,Jain DP, Cheng Y, Hardison RC, and Blobel GA. Tissue-specific Mitotic Bookmarking by Hematopoietic Transcription Factor GATA1. Cell. Vol 150. 2012:725.